|
KMID : 0894520060100020115
|
|
Development & Reproduction
2006 Volume.10 No. 2 p.115 ~ p.125
|
|
|
Effect of Selenium on Oocyte Maturation and Viability in vitro in Mouse
|
|
Choi Eun-Jin
Hong Soon-Gab
Kim Hae-Kwon
Yoon Yong-Dal
Lee Jun-Young
|
|
|
Abstract
|
|
|
|
The present experiment was performed to confirm the effects of selenium on maturation and viability of mouse oocyte. Maturation of oocytes was observed by microscope, Germinal vesicle breakdown(GVBD) and polar body formation(PB) were confirmed at 2.5, 13 hours after in vitro culture. Viability of oocytes was observed by microscope. Normal and abnormal oocytes were distinguished by morphological change in vitro culture for 72 hours. Glutathione(GSH) content of collected oocytes from individual stage also was measured by glutathione assay using spectrophotometer. The results obtained were as follows; The low concentration of selenium(0.005㎍/mL～0.5㎍/mL) increased the maturation rate of germinal vesicle(GV) oocytes to GVBD and PB oocytes. The high concentration of selenium(5㎍/mL) decreased the maturation rate. The low concentration of selenium increased the viability rate of PB oocytes. The high concentration of selenium did not affect the viability rate. The low concentration of selenium increased the GSH content in PB oocytes. The high concentration of selenium decreased GSH content. GSH content in PB oocyte was much higher than that in GVBD oocyte. The results indicate that the low concentration of selenium increases the maturation rate by helping quality elevation of oocyte and minimizing damages of oxidative stress generated from metabolism process. The low concentration of selenium also increases the viability rate by increasing GSH content.
|
|
|
KEYWORD
|
|
|
Mouse oocyte, Maturation, Viability, Selenium, Glutathione
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|